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梁子才 教授 阅读次数:

梁子才 教授

Ph.D.,博士生导师

核酸技术研究室主任

联系方式:010-6276-0308

E-mailliangz@pku.edu.cn

通讯地址:澳门新葡8455最新网站-点击进入新生命科学大楼213室 100871

 

1981-1988年就读南开大学生物系,1985年获学士学位;1988年获硕士学位;1990-1995年就读瑞典乌普萨拉大学(Uppsala University)并获博士学位。1988-1990年到中国科学院动物澳门新葡8455,任实习研究员;1995-1998年在美国耶鲁大学生化生物物理学系从事博士后研究;1998-2005年任瑞典卡珞琳斯卡医学院基因组与生物信息学中心基因组技术研究室主任, 助教授,副教授;2006年至任澳门新葡8455最新网站教授,博士研究生导师,核酸技术研究室主任。同时兼任www.8455.com教育委员会主任,学术委员会副主任;澳门新葡8455最新网站-点击进入生物学科学位委员会副主任。第一届、第二届、第三届中国小核酸技术与应用学术会议(RNAi China, 2008; RNAi Asia, 2009 &RNAi China, 2009; RNAi China, 2011)筹备人,组委会秘书长。2010年至今为中国生物化学与分子生物学学会核糖核酸专业委员会副主任委员。

梁子才教授主要从事RNA干扰领域基础和应用研究,在RNA干扰载体、siRNA特异性、稳定化等方面取得了一系列创新成果,获得了一批具有自主知识产权的核心技术,建立了一个我国的小核酸应用基础研究平台。其中包括:1)运用自有的MAST和siQuant研究方法,首次发表了一个小核酸在其所有单碱基突变的近似靶位上的脱靶效应图谱,完成了对小核酸在双碱基突变的近似靶位点的特异性和脱靶效应的研究,全方位比较RNA干扰过程中的碱基识别特异性在国际上第一次构建了RNA干扰中不同碱基在小核酸不同位置的碱基选择性图谱。这一系列原创性的系统、深入的创新发现对开展小核酸的基础研究和小核酸应用研究具有重要的理论意义。2)在国际上率先发明了双对向启动子驱动的小核酸表达载体,并实现了用这一载体一次性形成上亿种不同小核酸载体的试验。这一工作我国拥有完整的知识产权,与发卡式小核酸表达载体并列为世界上两种小核酸表达载体之一。相关专利在澳大利亚已经获得授权。3)在国际上率先开展了microRNA 22的功能研究,首次发现了microRNA 22与c-Myc和MYCBP形成调控回路,揭示microRNA 22在肿瘤发生发展中的作用。将对microRNA调控肿瘤发生发展的机制研究是一个贡献。4)剖析揭示了双链RNA降解的序列特异性规律,进一步展示了利用这一规律指导小核酸稳定化的可行性。这一研究不仅填补了小核酸以及所有双链RNA代谢过程的空白,也为核酸稳定化提供了理论基础。5)在国际上第一个发现并阐明siRNA在动物体内的一条新的代谢清除途径:肝肠清除途径。填补了siRNA自身体内行为的“另一半”空白,并开展多枝体(dendrimer)和纳米粒(nanoparticle)的合作研究。6)开发了中国第一个细胞电穿孔技术体系用于DNA和siRNA delivery。

 

代表论文

Year 2016

1. Meng X, Jiang Q, Chang N, Wang X, Liu C, Xiong J, Cao H*, Liang Z*. Small activating RNA binds to the genomic target site in a seed-region-dependent manner. Nucleic Acids Res. 2016, 44(5):2274-2282.

2. Zhou X, Han X, Wittfeldt A, Sun J, Liu C, Wang X, Gan LM*, Cao H*, Liang Z*. Long non-coding RNA ANRIL regulates inflammatory responses as a novel component of NF-κB pathway. RNA Biol. 2016,13(1):98-108.

3. Cheng Q, Du L, Meng L, Han S, Wei T, Wang X, Wu Y, Song X, Zhou J, Zheng S, Huang Y, Liang XJ, Cao H, Dong A*, Liang Z*. The Promising Nanocarrier for Doxorubicin and siRNA Co-delivery by PDMAEMA-based Amphiphilic Nanomicelles. ACS Appl Mater Interfaces. 2016, 8(7):4347-4356.

4. Huang Y, Cheng Q, Jin X, Ji JL, Guo S, Zheng S, Wang X, Cao H, Gao S, Liang XJ, Du Q*, Liang Z*. Systemic and tumor-targeted delivery of siRNA by cyclic NGR and isoDGR motif-containing peptides. Biomater Sci. 2016,4(3):494-510.

5. Zhao D, Huang D, Li Y, Wu M, Zhong W, Cheng Q, Wang X, Wu Y, Zhou X, Wei Z, Li Z*, Liang Z*. A Flow-Through Cell Electroporation Device for Rapidly and Efficiently Transfecting Massive Amounts of Cells in vitro and ex vivo. Sci Rep. 2016, 6:18469.

6. Liu J, Wei T, Zhao J, Huang Y, Deng H, Kumar A, Wang C, Liang Z, Ma X, Liang XJ. Multifunctional aptamer-based nanoparticles for targeted drug delivery to circumvent cancer resistance. Biomaterials. 2016, 91:44-56.

7. Li D, Liu J, Guo B, Liang C, Dang L, Lu C, He X, Cheung HY, Xu L, Lu C, He B, Liu B, Shaikh AB, Li F, Wang L, Yang Z, Au DW, Peng S, Zhang Z, Zhang BT, Pan X, Qian A, Shang P, Xiao L, Jiang B, Wong CK, Xu J, Bian Z, Liang Z, Guo DA, Zhu H, Tan W, Lu A, Zhang G. Osteoclast-derived exosomal miR-214-3p inhibits osteoblastic bone formation. Nat Commun. 2016,7:10872.

8. Zhou J, Li D, Wen H, Zheng S, Su C, Yi F, Wang J, Liang Z, Tang T, Zhou D, Zhang LH, Liang D, Du Q. Inter-molecular β-sheet structure facilitates lung-targeting siRNA delivery. Sci Rep. 2016, 6:22731.

Year 2015

9. Han X, Yang F, Cao H, Liang Z. Malat1 regulates serum response factor through miR-133 as a competing endogenous RNA in myogenesis. FASEB J. 2015,29(7):3054-3064.

10. Han S, Cheng Q, Wu Y, Zhou J, Long X, Wei T, Huang Y, Zheng S, Zhang J, Deng L, Wang X, Liang XJ, Cao H, Liang Z*, Dong A*. Effects of hydrophobic core components in amphiphilic PDMAEMA nanoparticles on siRNA delivery. Biomaterials. 2015,48:45-55.

11. Liu J, Dang L, Li D, Liang C, He X, Wu H, Qian A, Yang Z, Au DW, Chiang MW, Zhang BT, Han Q, Yue KK, Zhang H, Lv C, Pan X, Xu J, Bian Z, Shang P, Tan W, Liang Z, Guo B, Lu A, Zhang G. A delivery system specifically approaching bone resorption surfaces to facilitate therapeutic modulation of microRNAs in osteoclasts. Biomaterials. 2015,52:148-160.

12. Wei T, Chen C, Liu J, Liu C, Posocco P, Liu X, Cheng Q, Huo S, Liang Z, Fermeglia M, Pricl S, Liang XJ, Rocchi P, Peng L. Anticancer drug nanomicelles formed by self-assembling amphiphilic dendrimer to combat cancer drug resistance. Proc Natl Acad Sci U S A. 2015 ,112(10):2978-2983.

13. Liang C, Guo B, Wu H, Shao N, Li D, Liu J, Dang L, Wang C, Li H, Li S, Lau WK, Cao Y, Yang Z, Lu C, He X, Au DW, Pan X, Zhang BT, Lu C, Zhang H, Yue K, Qian A, Shang P, Xu J, Xiao L, Bian Z, Tan W, Liang Z, He F, Zhang L, Lu A, Zhang G. Aptamer-functionalized lipid nanoparticles targeting osteoblasts as a novel RNA interference-based bone anabolic strategy. Nat Med. 2015 ,21(3):288-294.

14. Li Y, Wu M, Zhao D, Wei Z, Zhong W, Wang X, Liang Z*, Li Z*. Electroporation on microchips: the harmful effects of pH changes and scaling down. Sci Rep. 2015, 5:17817.

15. Zhang L, Xu Y, Jin X, Wang Z, Wu Y, Zhao D, Chen G, Li D, Wang X, Cao H, Xie Y, Liang Z*. A circulating miRNA signature as a diagnostic biomarker for non-invasive early detection of breast cancer. Breast Cancer Res Treat. 2015,154(2):423-434.

16. Huang Y, Wang X, Huang W, Cheng Q, Zheng S, Guo S, Cao H, Liang XJ, Du Q*, Liang Z*. Systemic Administration of siRNA via cRGD-containing Peptide. Sci Rep. 2015,5:12458.

17. Zhang L, Song X, Wang X, Xie Y, Wang Z, Xu Y, You X, Liang Z, Cao H. Circulating DNA of HOTAIR in serum is a novel biomarker for breast cancer. Breast Cancer Res Treat. 2015 ,152(1):199-208.

18. Liang C, Li D, Zhang G, Li H, Shao N, Liang Z, Zhang L, Lu A, Zhang G. Comparison of the methods for generating single-stranded DNA in SELEX. Analyst. 2015, 140(10):3439-3444.

19. He YX, Liu J, Guo B, Wang YX, Pan X, Li D, Tang T, Chen Y, Peng S, Bian Z, Liang Z, Zhang BT, Lu A, Zhang G. Src inhibitor reduces permeability without disturbing vascularization and prevents bone destruction in steroid-associated osteonecrotic lesions in rabbits. Sci Rep. 2015,5:8856.

20. Yang Z, Cheng Q, Jiang Q, Deng L, Liang Z*, Dong A*. Thermo-sensitive nanoparticles for triggered release of siRNA. J Biomater Sci Polym Ed. 2015,26(4):264-276.

21. Wei Z, Huang Y, Zhao D, Hu Z*, Li Z*, Liang Z*. A pliable electroporation patch (ep-Patch) for efficient delivery of nucleic acid molecules into animal tissues with irregular surface shapes. Sci Rep. 2015 ,5:7618.

Year 2014

22. Li Y, Cheng Q, Jiang Q, Huang Y, Liu H, Zhao Y, Cao W, Ma G, Dai F, Liang X, Liang Z*, Zhang X*. Enhanced endosomal/lysosomal escape by distearoyl phosphoethanolamine -polycarboxybetaine lipid for systemic delivery of siRNA. J Control Release. 2014 ,176:104-114

23. Wei Z, Li X, Zhao D, Yan H, Hu Z*, Liang Z*, Li Z*. Flow-through cell electroporation microchip integrating dielectrophoretic viable cell sorting. Anal Chem. 2014,86(20):10215-10222.

24. Wei Z, Zheng S, Wang R, Bu X, Ma H, Wu Y, Zhu L, Hu Z*, Liang Z*, Li Z*. A flexible microneedle array as low-voltage electroporation electrodes for in vivo DNA and siRNA delivery. Lab Chip. 2014 ,14(20):4093-4102.

25. Jiang Q, Meng X, Meng L, Chang N, Xiong J, Cao H*, Liang Z*. Small indels induced by CRISPR/Cas9 in the 5' region of microRNA lead to its depletion and Drosha processing retardance. RNA Biol. 2014,(10):1243-1249.

26. Li Y, Zheng S, Liang X, Jin Y, Wu Y, Bai H, Liu R, Dai Z*, Liang Z*, Shi T. Doping hydroxylated cationic lipid into PEGylated cerasome boosts in vivo siRNA transfection efficacy. Bioconjug Chem. 2014 ,25(11):2055-2066.

27. Liu X, Zhou J, Yu T, Chen C, Cheng Q, Sengupta K, Huang Y, Li H, Liu C, Wang Y, Posocco P, Wang M, Cui Q, Giorgio S, Fermeglia M, Qu F, Pricl S, Shi Y, Liang Z, Rocchi P, Rossi JJ, Peng L. Adaptive amphiphilic dendrimer-based nanoassemblies as robust and versatile siRNA delivery systems. Angew Chem Int Ed Engl. 2014,53(44):11822-11827. doi: 10.1002/anie.201406764. Epub 2014 Sep 12.

28. Feng Y, Fan Y, Huiqing C, Zicai L*, Quan D*. The emerging landscape of long non-coding RNAs. Yi Chuan. 2014 36(5):456-468.

29. Huang W, Zhao M, Wei N, Wang X, Cao H, Du Q *, Zicai Liang*. Site-specific RNase A activity was dramatically reduced in serum from multiple types of cancer patients. Plos One.2014,9(5):e96490

Year 2013

30. Cheng Q, Huang Y*, Zheng H, Wei T, Zheng S, Huo S, Wang X, Du Q, Zhang X, Zhang HY, Liang XJ, Wang C, Tang R*, Liang Z*. The effect of guanidinylation of PEGylated poly(2-aminoethyl methacrylate) on the systemic delivery of siRNA. Biomaterials. 2013,34(12):3120-3131.

31. Zhang W, Cheng Q, Guo S, Lin D, Huang P, Liu J, Wei T, Deng L, Liang Z*, Liang XJ*, Dong A*. Gene transfection efficacy and biocompatibility of polycation/DNA complexes coated with enzyme degradable PEGylated hyaluronic acid. Biomaterials. 2013 ,34(27):6495-6503.

32. Zheng J, Zhang L, Zhang J, Wang X, Ye K, Xi Z*, Du Q*, Liang Z*.Single modification at position 14 of siRNA strand abolishes its gene-silencing activity by decreasing both RISC loading and target degradation. FASEB J. 2013 ,27(10):4017-4026.

33. Yi F, Yang F, Liu X, Chen H, Ji T, Jiang L, Wang X, Yang Z, Zhang LH, Ding X, Liang Z*, Du Q*. RNA-seq identified a super-long intergenic transcript functioning in adipogenesis. RNA Biol. 2013 ,10(6):991-1001.

34. Lin D, Jiang Q, Cheng Q, Huang Y, Huang P, Han S, Guo S, Liang Z*, Dong A*. Polycation-detachable nanoparticles self-assembled from mPEG-PCL-g-SS-PDMAEMA for in vitro and in vivo siRNA delivery. Acta Biomater. 2013 ,9(8):7746-7757.

35. Lin D, Cheng Q, Jiang Q, Huang Y, Yang Z, Han S, Zhao Y, Guo S, Liang Z*, Dong A*. Intracellular cleavable poly(2-dimethylaminoethyl methacrylate) functionalized mesoporous silica nanoparticles for efficient siRNA delivery in vitro and in vivo. Nanoscale. 2013 ,5(10):4291-4301.

36. Wu M, Zhao D, Wei Z, Zhong W, Yan H, Wang X, Liang Z*, Li Z*. Method for Electric Parametric Characterization and Optimization of Electroporation on a Chip. Anal Chem. 2013,85(9):4483-4491.

37. Yang F, Yi F, Han X, Du Q*, Liang Z*. MALAT-1 interacts with hnRNP C in cell cycle regulation. FEBS Lett. 2013 ,587(19):3175-3181.

38. Wei T, Liu J, Ma H, Cheng Q, Huang Y, Zhao J, Huo S, Xue X, Liang Z. Liang XJ, Functionalized nanoscale micelles improve the drug delivery for cancer in vitro and in vivo. Nano Lett. 2013 ,13(6):2528-2534.

39. Wu M, Zhao D, Zhong W, Yan H, Wang X, Liang Z*, Li Z*. High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes. Sci Rep. 2013,3:3370.

40. Guo B, Zhang B, Zheng L, Tang T, Liu J, Wu H, Yang Z, Peng S, He X, Zhang H, Yue KK, He F, Zhang L, Qin L, Bian Z, Tan W, Liang Z, Lu A, Zhang G. Therapeutic RNA interference targeting CKIP-1 with a cross-species sequence to stimulate bone formation. Bone. 2014 ,59:76-88.

41. Liang Z*, Wang XJ*. Rising from Ashes: Non-Coding RNAs Come of Age. J Genet Genomics. 2013,40(4):141-142.

Year 2012

42. Wei N, Zhang L, Huang H, Chen Y, Zheng J, Zhou X, Yi F, Du Q*, Liang Z*. siRNA Has Greatly Elevated Mismatch Tolerance at 3'-UTR Sites. PLoS One. 2012, 7(11):e49309.

43. Peschansky VJ, Liang Z, Wahlestedt C. RNAi Joins the "Singles Club". Mol Ther. 2012 , 20(11):2010-2011. doi: 10.1038/mt.2012.218.

44. Zhang J, Zheng J, Lu C, Du Q, Liang Z*, Xi Z*. Modification of the siRNA passenger strand by 5-nitroindole dramatically reduces its off-target effects. Chembiochem. 2012 ,13(13):1940-1945.

45. Huang Y, Lin D, Jiang Q, Zhang W, Guo S, Xiao P, Zheng S, Wang X, Chen H, Zhang HY, Deng L, Xing J, Du Q, Dong A*, Liang Z*. Binary and ternary complexes based on polycaprolactone-graft-poly (N, N-dimethylaminoethyl methacrylate) for targeted siRNA delivery. Biomaterials. 2012 ,33(18):4653-4664.

46. Ding X, Ding J, Ning J, Yi F, Chen J, Zhao D, Zheng J, Liang Z*, Hu Z*, Du Q*. Circulating microRNA-122 as a potential biomarker for liver injury. Mol Med Report. 2012, 5(6):1428-1432.

47. Yang F, Yi F, Zheng Z, Ling Z,Ding J, Guo J, Mao W, Wang X, Wang X, Ding X, Liang Z*,Du Q*. Characterization of a carcinogenesis-associated long non-coding RNA. RNA Biol. 2012 , 9(1):110-116.

Year 2011

48. Huang Y, Hong J, Zheng S, Ding Y, Guo S, Zhang H, Zhang X, Du Q*, Liang Z*. Elimination pathways of systemically delivered siRNA. Mol Ther. 2011, 19(2): 381-385.

49. Lin D, Huang Y, Jiang Q, Zhang W, Yue X, Guo S, Xiao P, Du Q, Xing J, Deng L, Liang Z*, Dong A*. Structural contributions of blocked or grafted poly(2-dimethylaminoethyl methacrylate) on PEGylated polycaprolactone nanoparticles in siRNA delivery. Biomaterials. 2011, 32(33): 8730-8742.

50. Wei Z, Zhao D, Li X, Wu M, Wang W, Huang H, Wang X, Du Q, Liang Z*, Li Z*. A laminar flow electroporation system for efficient DNA and siRNA delivery. Anal Chem. 2011, 83(15): 5881-5887.

51. Guo S, Huang Y, Zhang W, Wang W, Wei T, Lin D, Xing J, Deng L, Du Q, Liang Z*, Liang XJ*, Dong A*. Ternary complexes of amphiphilic polycaprolactone-graft-poly (N,N-dimethylaminoethyl methacrylate), DNA and polyglutamic acid-graft-poly(ethylene glycol) for gene delivery. Biomaterials. 2011, 32(18): 4283-4292.

52. Huang H, Wei Z, Huang Y, Zhao D, Zheng L, Cai T, Wu M, Wang W, Ding X, Zhou Z, Du Q*, Li Z*, Liang Z*. An efficient and high-throughput electroporation microchip applicable for siRNA delivery. Lab Chip. 2011, 11(1): 163-172.

53. Zhou J, Neff CP, Liu X, Zhang J, Li H, Smith DD, Swiderski P, Aboellail T, Huang Y, Du Q, Liang Z, Peng L, Akkina R, Rossi JJ. Systemic Administration of Combinatorial dsiRNAs via Nanoparticles Efficiently Suppresses HIV-1 Infection in Humanized Mice. Mol Ther. 2011,19(12):2228-2238.

54. Lu Y, Zhang J, Xiong Y, Zhu N, Fan C, Wang S, Liu S, Liang Z, Shen Y, Wang Q, Chen M. Small interfering RNAs screened from random siRNA library direct neuronal differentiation. Biotechnol Lett. 2011, 33(9): 1737-1744.

55. Fan C, Xiong Y, Zhu N, Lu Y, Zhang J, Wang S, Liang Z, Shen Y, Chen M. Random small interfering RNA library screen identifies siRNAs that induce human erythroleukemia cell differentiation. Leuk Lymphoma. 2011, 52(3): 502-514.

56. Guo S, Huang Y, Wei T, Zhang W, Wang W, Lin D, Zhang X, Kumar A, Du Q, Xing J, Deng L, Liang Z, Wang PC, Dong A, Liang XJ. Amphiphilic and biodegradable methoxy polyethylene glycol-block- (polycaprolactone- graft-poly (2-(dimethylamino) ethyl methacrylate)) as an effective gene carrier. Biomaterials. 2011, 32(3): 879-889.

Year 2010 and beyond

57. Hong J, Huang Y, Li J, Yi F, Zheng J, Huang H, Wei N, Shan Y, An M, Zhang H, Ji J, Zhang P, Xi Z*, Du Q*, Liang Z*. Comprehensive analysis of sequence-specific stability of siRNA. FASEB J. 2010,24(12):4844-4855.

58. Xiong J, Du Q, Liang Z*. Tumor-suppressive microRNA-22 inhibits the transcription of E-box-containing c-Myc target genes by silencing c-Myc binding protein. Oncogene. 2010, 29(35):4980-4988.

59. Xiong J, Yu D, Wei N, Fu H, Cai T, Huang Y, Wu C, Zheng X, Du Q*, Lin D*, Liang Z*. An estrogen receptor alpha suppressor, microRNA-22, is downregulated in estrogen receptor alpha-positive human breast cancer cell lines and clinical samples. FEBS J. 2010, 277(7):1684-1694.

60. Liang Z, Zhang P. RNA interference (RNAi) technology. Comprehensive Biotechnology, 2nd edition, chapter 342.

61. Du Q, Huang H, Liang Z. siRNA: The specificity and off-target effects. Medicinal chemistry of nucleic acids. 2011. DOI: 10.1002/9781118092804.ch12

62. Wang X, Yan F, Du Q, Liang Z*. siRNAs as potential drugs. Drug Discovery Today: Technologies 7. 2010, Pages e125-e130 doi:10.1016/j.ddtec.2010.11.011

63. Xiong X, Lu Y, Zhang L, Wang B, Zhao Y, Wang XJ, Huo X, Shen Y*, Liang Z*, Chen M*. Discovery of novel cell proliferation-enhancing gene by random siRNA library based combinatorial screening. Comb Chem High Throughput Screen. 2010, 13(9):798-806.

64. Guo S, Huang Y, Jiang Q, Sun Y, Deng L, Liang Z, Du Q, Xing J, Zhao Y, Wang PC, Dong A, Liang XJ. Enhanced gene delivery and siRNA silencing by gold nanoparticles coated with charge-reversal polyelectrolyte. ACS Nano. 2010, 4(9):5505-5511.

65. Huang H, Wei N, Xiong Y, Yang F, Fang H, Xie W, Zhou Z, Cheng H, Liang Z*, Du Q*. Exogenous nucleic acids aggregate in non-P-body cytoplasmic granules when transfected into cultured cells. Front Biol. 2010, 5, 272-281.

66. Huang H, Qiao R, Zhao D, Zhang T, Li Y, Yi F, Lai F, Hong J, Ding X, Yang Z, Zhang L, Du Q*, Liang Z*. Profiling of mismatch discrimination in RNAi enabled rational design of allele-specific siRNAs. Nucleic Acids Res. 2009, 37(22):7560-7569.

67. Li J, Liang Z. The consideration of synthetic short interfering RNA for therapeutic use. Basic Clin Pharmacol Toxicol. 2010, 106(1):22-29.

68. Xue Y, Petrovic N, Cao R, Larsson O, Lim S, Chen S, Feldmann HM, Liang Z, Zhu Z, Nedergaard J, Cannon B, Cao Y. Hypoxia-independent angiogenesis in adipose tissues during cold acclimation. Cell Metab. 2009, 9(1):99-109.

69. Liu XX, Rocchi P, Qu FQ, Zheng SQ, Liang ZC, Gleave M, Iovanna J, Peng L. PAMAM dendrimers mediate siRNA delivery to target Hsp27 and produce potent antiproliferative effects on prostate cancer cells. ChemMedChem. 2009,4(8):1302-1310.

70. Dahlgren C, Zhang HY, Du Q, Grahn M, Norstedt G, Wahlestedt C, Liang Z*. Analysis of siRNA specificity on targets with double-nucleotide mismatches. Nucleic Acids Res. 2008,36(9):e53.

71. Hong J, Wei N, Chalk A, Wang J, Song Y, Yi F, Qiao RP, Sonnhammer EL, Wahlestedt C, Liang Z*, Du Q*. Focusing on RISC assembly in mammalian cells. Biochem Biophys Res Commun. 2008, 368(3):703-708.

72. Li ZS, Qiao RP, Du Q, Yang ZJ, Zhang LR, Zhang PZ, Liang ZC, Zhang LH. Studies on aminoisonucleoside modified siRNAs: stability and silencing activity. Bioconjug Chem. 2007,18(4):1017-1024.

73. Chen M, Du Q, Zhang HY, Wang X, Liang Z*. High-throughput screening using siRNA (RNAi) libraries. Expert Rev Mol Diagn. 2007, 7(3):281-291.

74. Zaslavsky A, Singh LS, Tan H, Ding H, Liang Z, Xu Y. Homo- and hetero-dimerization of LPA/S1P receptors, OGR1 and GPR4. Biochim Biophys Acta. 2006, 1761(10):1200-1212.

75. Larsson O, Perlman DM, Fan D, Reilly CS, Peterson M, Dahlgren C, Liang Z, Li S, Polunovsky VA, Wahlestedt C, Bitterman PB. Apoptosis resistance downstream of eIF4E: posttranscriptional activation of an anti-apoptotic transcript carrying a consensus hairpin structure. Nucleic Acids Res. 2006, 34(16):4375-4386.

76. Gao Y, Sun L, Dong J, Xu X, Shu Y, Chen M, Yin L, Liang Z*, Jin Q*. Rapid identification of small interfering RNA that can effectively inhibit the replication of multiple influenza B virus strains. Antivir Ther. 2006, 11(4):431-438.

77. Zhang HY, Du Q, Wahlestedt C, Liang Z*. RNA Interference with chemically modified siRNA. Curr Top Med Chem. 2006, 6(9):893-900.

78. Chen M, Zhang HY, Wahlestedt C, Liang Z*. siRNA based High throughput screening. European Pharmaceutical Review, 2006, 11:13-17.

79. Liang Z*. siRNA based High throughput screening. Idrugs. 2005, 8:924-926.

80. Du Q*, Thonberg H, Wang J, Wahlestedt C, Liang Z*. A systematic analysis of the silencing effects of an active siRNA at all single-nucleotide mismatched target sites. Nucleic Acids Res. 2005, 33(5):1671-1677.

81. Chen M, Zhang L, Zhang HY, Xiong X, Wang B, Du Q, Lu B, Wahlestedt C, Liang Z*. A universal plasmid library encoding all permutations of small interfering RNA. Proc Natl Acad Sci U S A. 2005, 102(7):2356-2361.

82. Elmén J, Thonberg H, Ljungberg K, Frieden M, Westergaard M, Xu Y, Wahren B, Liang Z, ?rum H, Koch T, Wahlestedt C. Locked nucleic acid (LNA) mediated improvements in siRNA stability and functionality. Nucleic Acids Res. 2005 ,33(1):439-447.

83. Chen M, Du Q, Zhang HY, Wahlestedt C, Liang Z*. Vector-based siRNA delivery strategies for high-throughput screening of novel target genes. J RNAi Gene Silencing. 2005 ,1(1):5-11.

84. Elmén J, Zhang HY, Zuber B, Ljungberg K, Wahren B, Wahlestedt C, Liang Z. Locked nucleic acid containing antisense oligonucleotides enhance inhibition of HIV-1 genome dimerization and inhibit virus replication. FEBS Lett. 2004, 578(3):285-290.

85. Katayama S, Tomaru Y, Kasukawa T, Waki K, Nakanishi M, Nakamura M, Nishida H, Yap CC, Suzuki M, Kawai J, Suzuki H, Carninci P, Hayashizaki Y, Wells C, Frith M, Ravasi T, Pang KC, Hallinan J, Mattick J, Hume DA, Lipovich L, Batalov S, Engstr?m PG, Mizuno Y, Faghihi MA, Sandelin A, Chalk AM, Mottagui-Tabar S, Liang Z, Lenhard B, Wahlestedt C; RIKEN Genome Exploration Research Group; Genome Science Group (Genome Network Project Core Group); FANTOM Consortium. Antisense transcription in the mammalian transcriptome. Science. 2005 ,309(5740):1564-1566.

86. Du Q, Larsson O, Swerdlow H, Liang Z*. DNA immobilization: silanized nucleic acids and nanoprinting. Topics in Curr Chemistry. 2005, 261:45-61.

87. Du Q, Thonberg H, Zhang HY, Wahlestedt C, Liang Z*. Validating siRNA using a reporter made from synthetic DNA oligonucleotides. Biochem Biophys Res Commun. 2004, 325(1):243-249.

88. Xu Y, Linde A, Larsson O, Thormeyer D, Elmen J, Wahlestedt C, Liang Z*. Functional comparison of single- and double-stranded siRNAs in mammalian cells. Biochem Biophys Res Commun. 2004, 316(3):680-687.

89. Larsson O*, Scheele C, Liang Z*, Moll J, Karlsson C, Wahlestedt C. Kinetics of senescence-associated changes of gene expression in an epithelial, temperature-sensitive SV40 large T antigen model. Cancer Res. 2004, 64(2):482-489.

90. Zhang HY, Mao J, Zhou D, Xu Y, Thonberg H, Liang Z*, Wahlestedt C*. mRNA accessible site Tagging(MAST): a novel method of selecting effective antisense oligonucleotides. Nucleic Acids Res. 2003, 15;31(14):e72.

91. Xu Y, Zhang HY, Thormeyer D, Larsson O, Du Q, Elmén J, Wahlestedt C, Liang Z*. Effective small interfering RNAs and phosphorothioate antisense DNAs have different preferences for target sites in the luciferase mRNAs. Biochem Biophys Res Commun. 2003, 306(3):712-717.

92. Thormeyer D, Ammerpohl O, Larsson O, Xu Y, Asinger A, Wahlestedt C, Liang Z*. Characterization of lacZ complementation deletions using membrane receptor dimerization. BioTechniques. 2003, 34(2):346-350,352-355.

93. Larsson O, Thormeyer D, Asinger A, Wihlén B, Wahlestedt C, Liang Z*. Quantitative codon optimisation of DNA libraries encoding sub-random peptides: design and characterisation of a novel library encoding transmembrane domain peptides. Nucleic Acids Res. 2002, 30(23):e133.

94. Kumar A, Liang Z*. Chemical nanoprinting: a novel method for fabricating DNA microchips. Nucleic Acids Res. 2001, 29(2):E2.

95. Kumar A, Larsson O, Parodi D, Liang Z*. Silanized nucleic acids: a general platform for DNA immobilization. Nucleic Acids Res. 2000, 28(14):E71.

96. Wang R, Liang Z, Hal M, S?derh?ll K. A transglutaminase involved in the coagulation system of the freshwater crayfish, Pacifastacus leniusculus. Tissue localisation and cDNA cloning. Fish Shellfish Immunol. 2001, 11(7):623-637.

97. Liang Z, Biggin M.D. Eve and ftz regulate a wide array of genes in blastoderm embryos: the selector homeoproteins directly or indirectly regulate most genes in Drosophila. Development. 1998, 125:4471-4482.

98. Liang Z, Sottrup-Jensen L, Aspán A, Hall M, S?derh?ll K. Pacifastin, a novel 155-kDa heterodimeric proteinase inhibitor containing a unique transferrin chain. Proc. Natl. Acad. Sci. 1997, 94:6682-6687.

99. Liang Z, S?derh?ll K. Isolation of cDNA encoding a novel serpin of crayfish hemocytes. Comp. Biochem. Physiol. 1995, B112(2):385-391.

100. Cerenius L, Liang Z, Duvic B, Keyser P, Hellman U, Palva E.T, Iwanaga S, S?derh?ll K. Structure and biological activity of a 1,3-beta-D-glucan-binding protein in crustacean blood. J. Biol Chem. 1994, 269:29462-29467.

101. Liang Z, Lindblad P, Beauvais A, Johansson M.W, Latgé J, Hall M, Cerenius L, S?derh?ll K. Crayfish α-macroglobulin and 76 kDa protein; Their biosynthesis and subcellular localization of the 76 kDa protein. J. Insect Physiol. 1992, 38:987-995.

102. Rantamaki J, Durrant H, Liang Z, Ratcliffe N.A, Duvic B, S?derh?ll K. Isolation of a 90 kDa protein from haemocytes of Blaberus craniifer which has similar function and immunological properties to the 76 kDa protein from crayfish haemocytes. J. Insect Physiol. 1991, 37:627-634.

103. Cheng Z, Liang Z. Studies on the phenoloxidase in the hemolymph of the Asian cornborer Ostrinia furnacalis. Acta Entomologica Sinica. 1990, 33:424-429.

104. Liang Z, Cheng Z. Studies on the cellular defense reactions of the Asian cornborer Ostrinia furnacalis. Acta Entomologica Sinica. 1990, 33:430-440.