澳门新葡8455最新网站-点击进入

研究方向:

  研究室致力于研究器官(含心脏发育起源与再生的细胞与分子机制。我们的研究集中在如下两个方向:1. 胚胎期中胚层来源器官(含心脏干细胞特化与分化的表观遗传调控机制,以及先心病产生的细胞起源机理。我们利用/发展单细胞转录组与表观遗传学技术等手段,探索组织干细胞特化与命运决定的关键增强子组与其调控的转录因子网络。2. 实时成像结合数学模型绘制器官心脏发育起源/异常心脏再生的细胞学图谱。我们利用光片显微镜与图像图形处理技术,结合细胞谱系示踪,追踪心脏干细胞的增殖、分化、迁移和分配,绘制细胞图谱。本实验室综合运用发育生物学、表观遗传学、生物信息学、光片显微镜技术和数学建模等多学科交叉工具与方法,解决上述科学问题。

研究室成员

研究室主任(PI):何爱彬 研究员 Ph.D Email: ahe@pku.edu.cn

研究生: 艾珊珊、罗颖洁、李鑫、岳晏竹、余先红、熊海清、李晨、张婕婕、张幼东、王千昊、刘雅茜、边云堃

实习本科生学生:刘敏(四川大学)、李鑫(北大生科院)、廖远辉(北京工业大学)

研究室地址澳门新葡8455最新网站-点击进入综合科研楼220-221室,北京,100871.

实验室电话010-6274-4560

代表性科研论文:

1. Ai S, Xiong H, Li CC, Luo Y, Shi Q, Liu Y, Yu X, Li C and He A. Profiling chromatin states using single-cell itChIP-seq. Nature Cell Biology. 2019.

2. Wang Q, Xiong H, Ai S, Yu X, Liu Y, Zhang J and He A. CoBATCH for High-throughput Single-cell Epigenomic Profiling. Mol Cell. 2019.

3. Xiong H, Luo Y, Yue Y, Zhang J, Ai S, Li X, Wang X, Zhang YL, Wei Y, Li H, Hu X, Li C and He A. Single-Cell Transcriptomics Reveals Chemotaxis Mediated Intra-Organ Crosstalk During Cardiogenesis. Circ Res. 2019.

4. Li Y, Ai S, Yu X, Li C, Li X, Yue Y, Wei Y, Li CY# and He A#. Replication-Independent Histone Turnover Underlines the Epigenetic Homeostasis in Adult Heart. Circ Res. 2019.

5. Li Y, Li C, Li S, Peng Q, An NA, He A# and Li CY#. Human exonization through differential nucleosome occupancy. Proc Natl Acad Sci U S A. 2018;115:8817-8822. #, co-corresponding authors.

6. Ai S, Yu X, Li Y, Peng Y, Li C, Yue Y, Tao G, Li C-Y, Pu WT and He A. Divergent Requirements for EZH1 in Heart Development Versus Regeneration. Circ Res. 2017;121:106-112.

7. Ai S, Peng Y, Li C, Gu F, Yu X, Yue Y, Ma Q, Chen J, Lin Z, Zhou P, Xie H, Prendiville TW, Zheng W, Liu Y, Orkin SH, Wang DZ, Yu J, Pu WT#, He A#. EED orchestration of heart maturation through i nteraction with HDACs is H3K27me3-independent. Elife. 2017;6. #, co-corresponding authors.

8. He A#, Gu F, Hu Y, Ma Q, Yi Ye L, Akiyama JA, Visel A, Pennacchio LA, Pu WT#. Dynamic GATA4 enhancers shape the chromatin landscape central to heart development and disease. Nat Commun. 2014;5:4907. #, co-corresponding authors.

9. Chen JY, Shen QS, Zhou WZ, Peng J, He BZ, Li Y, Liu CJ, Luan X, Ding W, Li S, Chen C, Tan BC, Zhang YE, He A#, Li CY#. Emergence, Retention and Selection: A Trilogy of Origination for Functional De Novo Proteins from Ancestral LncRNAs in Primates. PLoS Genet. 2015;11:e1005391. #, co-corresponding authors.

10. He A, Shen X, Ma Q, Cao J, von Gise A, Zhou P, Wang G, Marquez VE, Orkin SH, Pu WT. PRC2 directly methylates GATA4 and represses its transcriptional activity. Genes Dev. 2012;26:37-42.

11. He A, Ma Q, Cao J, von Gise A, Zhou P, Xie H, Zhang B, Hsing M, Christodoulou DC, Cahan P, Daley GQ, Kong SW, Orkin SH, Seidman CE, Seidman JG, Pu WT. Polycomb Repressive Complex 2 Regulates Normal Development of the Mouse Heart. Circ Res. 2012;110:406-15.

12. He A, Kong SW, Ma Q, Pu WT. Co-occupancy by multiple cardiac transcription factors identifies transcriptional enhancers active in heart. Proc Natl Acad Sci U S A. 2011;108:5632-7.